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d7324 polyclonal sheep ab  (Biosynth Carbosynth)


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    Biosynth Carbosynth d7324 polyclonal sheep ab
    D7324 Polyclonal Sheep Ab, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab+d7324/us12534516-1765-1-5?v=Biosynth+Carbosynth
    Average 93 stars, based on 50 article reviews
    d7324 polyclonal sheep ab - by Bioz Stars, 2026-07
    93/100 stars

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    Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). <t>D7324-capture</t> ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.
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    Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). D7324-capture ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.

    Journal: Journal of Virology

    Article Title: A modification to heptad repeat 1 of gp41 improves yield and/or quality of soluble pre-fusion HIV-1 envelope glycoprotein trimers

    doi: 10.1128/jvi.00913-25

    Figure Lengend Snippet: Characterization of BG505 SOS.664-dPG and related Env proteins. ( A ). D7324-capture ELISA with unpurified D7324-tagged BG505 SOS.664, SOS.664-dPG, and BG505 SOSIP.664 trimers expressed in the supernatant of HEK293T cells. ( B ) Top: SEC curve of BG505 SOS.664-dPG produced in suspension 293S cells and purified using 2G12 affinity chromatography. Bottom: BN-PAGE analysis of the SEC fractions depicted above. ( C ) NS-EM analysis on 2G12/SEC-purified BG505 SOS.664-dPG.

    Article Snippet: The trimers were then immobilized via their D7324 tags for 2 h at room temperature on half-well 96-well plates (Greiner) precoated with Ab D7324 (Aalto Bioreagents) at 10 μg/mL in 0.1 M NaHCO3, pH 8.6 overnight ( ).

    Techniques: Enzyme-linked Immunosorbent Assay, Produced, Suspension, Purification, Affinity Chromatography